Viogene® Gel/PCR DNA Isolation Kit
High purity of DNA (A260/A280 1.8-1.9)
>95% primer and salts removal
Recover DNA fragments from standard of low-melting point agarose gels in TAE or TBE buffer
Elute DNA with just 15-30µl Elution buffer or ddH20
Recovery up to 90%
Preparation Time: 10-15 minutes
No sodium Iodide to interfere with subsequent reactions
No shearing of large DNA fragments
Principle
Viogene Gel/PCRTM DNA Isolation System contains a silica-gel-membrane for binding of DNA in high-salt buffer (pH 4.5~5.5) and elution of DNA with low-salt buffer (pH 7.0~9.0) or ddH2O. The purification procedure removes primers, nucleotides, enzyme, salts, ethidium bromide and other impurities from DNA samples even in agarose.
Downstream Applications
Viogene Gel/PCRTM DNA Isolation System provides reproducible yields of high-purity DNA suitable for use in most applications, including:
Automated fluorescent and radioactive sequencing & PCR
Restriction digestion & modifying enzymatic reaction
Ligation
labeling, & hybridization
|
Product |
cat.No |
Package |
Contents |
MSDS |
Catalog |
Protocol |
|
Gel/PCRTM DNA Isolation System |
GP1001 |
50preps |
50 GP Columns, |
|
|
|
|
GP1002 |
250preps |
250 GP Columns, |
|
|
|