Prestained Protein Ladder
• Two reference bands – red at ~75 kDa and green at ~25 kDa.
• Ready to use – no need to boil.
• 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
• 2 μl to 3 μl per well for general Western transferring
The Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights from 10 to 180 kDa. The ladder contains one red reference band at 75 kDa and one green band at 25 kDa. The Omics RGB Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready-to-use. Do not heat, dilute, or add reducing agent before loading.
Contents
0.2~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25°C), 2% SDS,
1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15% glycerol.
Storage
Stable at 25°C for 2 weeks, at 4°C for 3 months and at -20°C for 24 months
產品編號 : DPM006-0500